I have this message trying to scan and vectorize with my Fujitsu autofeed scanner:
16 bit Windows Subsystem
c:/Windows/System32/AUTOEXEC.NT. The system file is not suitable for running MS-DOS and Microsoft Windows applications. Choose ‘Close’ to terminate the application.
Hitting ‘Close’ will crash TBS2.5
more information: The scanner works fine with Photoshop using twain drivers on the same system.