I have problems in the program of “Toon Boom Animate pro”
1-after we close the software and reopen the file lost some layers
2-We use flash to draw backgrounds and incorporated in the "toon Boom"
this issue succeeded many time Then stopped working i do not know why

It’s hard for me to find out from your description why you might be losing layers. Did you get any error message when you opened the program? Did you try to open files from the PLE?